How can I set micropipette volume with decimal separators, for example 109.5 in p200?
$begingroup$
And how can I set:
- 150.1 in p200
- 871.3 in p1000
- 840.5 in p1000?
molecular-biology
edited 2 days ago
Laurel
1484
asked Jan 14 at 18:09
SaraSara
141
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$endgroup$
add a comment |
$begingroup$
And how can I set:
- 150.1 in p200
- 871.3 in p1000
- 840.5 in p1000?
molecular-biology
edited 2 days ago
Laurel
1484
asked Jan 14 at 18:09
SaraSara
141
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
$endgroup$
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
21 hours ago
add a comment |
$begingroup$
And how can I set:
- 150.1 in p200
- 871.3 in p1000
- 840.5 in p1000?
molecular-biology
edited 2 days ago
Laurel
1484
asked Jan 14 at 18:09
SaraSara
141
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
$endgroup$
And how can I set:
- 150.1 in p200
- 871.3 in p1000
- 840.5 in p1000?
molecular-biology
molecular-biology
edited 2 days ago
Laurel
1484
asked Jan 14 at 18:09
SaraSara
141
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
edited 2 days ago
Laurel
1484
asked Jan 14 at 18:09
SaraSara
141
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
edited 2 days ago
Laurel
1484
edited 2 days ago
Laurel
1484
edited 2 days ago
Laurel
1484
1484
asked Jan 14 at 18:09
SaraSara
141
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
asked Jan 14 at 18:09
SaraSara
141
asked Jan 14 at 18:09
SaraSara
141
141
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
New contributor
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
Sara is a new contributor to this site. Take care in asking for clarification, commenting, and answering.
Check out our Code of Conduct.
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
21 hours ago
add a comment |
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
21 hours ago
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
21 hours ago
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
21 hours ago
add a comment |
3 Answers
3
active
oldest
votes
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
1062
$endgroup$
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
$begingroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered 2 days ago
swbarnes2swbarnes2
3,544811
$endgroup$
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
362116
$endgroup$
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
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3 Answers
3
active
oldest
votes
3 Answers
3
active
oldest
votes
active
oldest
votes
active
oldest
votes
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
1062
$endgroup$
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
1062
$endgroup$
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
1062
$endgroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
1062
answered Jan 14 at 19:06
betelgeusebetelgeuse
1062
answered Jan 14 at 19:06
betelgeusebetelgeuse
1062
answered Jan 14 at 19:06
betelgeusebetelgeuse
1062
1062
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
2
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
$begingroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered 2 days ago
swbarnes2swbarnes2
3,544811
$endgroup$
add a comment |
$begingroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered 2 days ago
swbarnes2swbarnes2
3,544811
$endgroup$
add a comment |
$begingroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered 2 days ago
swbarnes2swbarnes2
3,544811
$endgroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered 2 days ago
swbarnes2swbarnes2
3,544811
answered 2 days ago
swbarnes2swbarnes2
3,544811
answered 2 days ago
swbarnes2swbarnes2
3,544811
answered 2 days ago
swbarnes2swbarnes2
3,544811
3,544811
add a comment |
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
362116
$endgroup$
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
362116
$endgroup$
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
362116
$endgroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
362116
edited Jan 15 at 1:55
answered Jan 14 at 21:17
CellCell
362116
answered Jan 14 at 21:17
CellCell
362116
answered Jan 14 at 21:17
CellCell
362116
362116
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
Sara is a new contributor. Be nice, and check out our Code of Conduct.
Sara is a new contributor. Be nice, and check out our Code of Conduct.
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$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
21 hours ago